FIGURE 4.

HBI‐8000 inhibits cardiac hypertrophy and ventricular fibrosis. (A, B) HE staining (Scale bar = 100 μm). (C) Relative expression of cardiac hypertrophy‐associated factor mRNA. (D) Masson staining in the upper half and PSR staining in the lower half (Scale bar = 100 μm). (E–I) Western blotting to detect the levels of myocardial fibrosis markers Col1, TGF‐β1, PCNA and ɑ‐SMA. (J–N) Levels of a‐SMA, Fn1, Col1, Col3 and TGF‐β1 were detected by qPCR. *p < 0.05, **p < 0.01, ***p < 0.001, ******p < 0.0001.