FIGURE 1.

Junctional protein‐associated with coronary artery disease (JCAD) expression was increased in mice after partial hepatectomy (PH). Male wild‐type (WT) mice at 8‐week‐old were subjected to PH. (A) Western blotting was conducted to detect p‐YAP, Yes‐associated protein (YAP), JCAD and cyclin D1 levels in liver samples after 70% PH at different time points, and β‐actin was used as an internal control. Ratio of p‐YAP over YAP was presented as fold change of p‐YAP. Densitometric quantification of imaging bands for JCAD and cyclin D1 were shown (n = 3, one‐way analysis of variance [ANOVA] with Tukey's honest significant difference [HSD] test). (B) Representative immunohistochemical staining of JCAD at different time points after PH. The positive area was indicated by black arrow. Scale bars, 50 μm. (C) JCAD and 5‐ethynyl‐2′‐deoxyuridine (EdU) were co‐stained in liver samples 2 days post PH. Blue: nucleus; green: EdU; red: JCAD. Scale bars, 25 μm. (D–H) Relative gene expression of Ccnb1 (cyclin B1), Ccnd1 (cyclin D1), Birc5, Ccn2 (CTGF) and Kirrel1 was detected by quantitative reverse transcriptase polymerase chain reaction (RT‐qPCR) with β‐actin as the internal control (n = 3, one‐way ANOVA with Tukey's HSD test). All data are presented as mean ± standard error of mean (SEM), ^* p < .05, ^** p < .01, ^*** p < .001 compared with control (Ctrl) group.