FIGURE 4.

RNA‐sequencing analysis of liver samples in wild‐type (WT) and junctional protein‐associated with coronary artery disease knockout (JCAD‐KO) mice 2 days post partial hepatectomy (PH). (A) Principal component analysis (PCA) was performed in RNA‐sequencing data of WT and JCAD‐KO mice 2 days post PH. (B) Heatmap of gene expression profiles post PH. (C) mRNA levels of genes involved in cell cycle were determined by reverse transcriptase polymerase chain reaction (RT‐PCR), and presented as relative expression levels using β‐actin as a house‐keeping gene control (n = 6, Student's t‐test). (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis demonstrated that the terms associated with the cell cycle and DNA replication are the most enriched in the differential genes. (E and F) Cell cycle genes characteristic of the S phase (n = 51, Student's t‐test) and G2‐M transition (n = 18, Student's t‐test) were significantly downregulated by JCAD‐KO, the median (middle line), 25th and 75th percentile (dot plot) was indicated. All data are presented as mean ± standard error of mean (SEM). ^** p < .01, ^*** p < .001 compared to WT mice.