Table 2.
LC–MS/MS parameters for the measured transitions of cyclic nucleotide monophosphates (cNMPs) and internal standard (IS).
| Name | Type | Precursor ion | Product ion | Retention time | Ion polarity | Collision energy |
|---|---|---|---|---|---|---|
| c-di-GMP | Target (cNMP) | 691.1 | 152.1 | 6.048 | Positive | 25 |
| GMP | Target (cNMP) | 364.1 | 152.1 | 5.699 | Positive | 25 |
| 2′,3’-Br-cGMP | 8-Br-cAMP (IS) | 424 | 230 | 7.680 | Positive | 25 |