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. 2024 Mar 7;15:1305097. doi: 10.3389/fmicb.2024.1305097

Table 2.

LC–MS/MS parameters for the measured transitions of cyclic nucleotide monophosphates (cNMPs) and internal standard (IS).

Name Type Precursor ion Product ion Retention time Ion polarity Collision energy
c-di-GMP Target (cNMP) 691.1 152.1 6.048 Positive 25
GMP Target (cNMP) 364.1 152.1 5.699 Positive 25
2′,3’-Br-cGMP 8-Br-cAMP (IS) 424 230 7.680 Positive 25