Table 6.
ΔrisS in non-modulating (Bvg+) conditions.
| Cluster | WT Non-Mod vs. Mod | ΔrisS Non-Mod vs. Mod | ΔrisAS Non-Mod vs. Mod | ΔrisS vs. WT Non-Mod | ΔrisAS vs. WT Non-Mod | RisA/RisS requirement for repression in Bvg + conditions | Description | Gene |
|---|---|---|---|---|---|---|---|---|
| a | Up | Up | Up | Down | Down | Vags dependent both RisA and RisS for full expression in non-modulating (Bvg+) conditions, but do not require RisA or RisS for maintaining an expected vag transcriptional response | bopN (T3SS) | |
| b | Up | NS | NS | Down | Down | Vags dependent both RisA and RisS for full expression in non-modulating (Bvg+) conditions and for maintaining an expected vag transcriptional response | CJ015_08855 (fimbrial protein) | |
| c | Up | Up | Up | NS | NS | Vags not regulated by RisAS during non-modulating (Bvg+) conditions and do not require RisA or RisS for maintaining an expected vag transcriptional response | FHA and PRN | |
| d | Down | NS | NS | Up | Up | Requires both RisA and RisS | Vrgs that require both RisA and RisS for maintaining an expected vrg transcriptional response and require both RisA and RisS for repression in non-modulating (Bvg+) conditions | tonB (siderophore) |
| d | Down | NS | NS | NS | Up | Requires only RisA | Vrgs that require both RisA and RisS for maintaining an expected vrg transcriptional response and require only RisA for repression in in non-modulating (Bvg+) conditions | livG (amino acid transport) |
| e | Down | Down | NS | Up | Up | Requires both RisA and RisS | Vrgs that do not require RisA or RisS for maintaining an expected vrg transcriptional response and require both RisA and RisS for repression in in non-modulating (Bvg+) conditions | flaA (flagellar protein) |
| e | Down | Down | NS | NS | Up | Requires only RisA | Vrgs that do not require RisAS for maintaining an expected vrg transcriptional response and require only RisA for modulation or repression in non-modulating (Bvg+) conditions | lapD (c-di-GMP receptor) |
Transcriptional response of genes within clusters identified from the comparisons used to evaluate the differential expression of the ΔrisS mutant in non-modulating (Bvg-) conditions. Yellow, indicates genes upregulated (Up) (log2fc > 1); blue, indicates genes downregulated (Down) (log2fc < −1); white, no significant change or NS in gene expression (log2fc < 1, but > −1).