TABLE 2.

Kinetic parameters of BAC C8 and BAC C10 uptake by hOCT1–3 and hMATE1/2K

Models were chosen based on examination of Eadie-Hofstee plots. The standard Michaelis-Menten equation (eq. 2) was fitted to the BAC C8 data, whereas a substrate-inhibition model (eq. 3) was fitted to the BAC C10 uptake kinetics. Results are represented as mean ± S.D. of the K_m, V_max, and K_i,[s] from three independent experiments.

Transporter	BAC C8		BAC C10
	Km (μM)	Vmax (pmol/mg protein/min)	Km (μM)	Vmax (pmol/mg protein/min)	Ki,[s] (μM)
hOCT1	8.7 ± 2.3	1091 ± 339	6.8 ± 3.8	1480 ± 163	93.1 ± 19.4
hOCT2	10.4 ± 3.9	965 ± 43	19.1 ± 6.8	2673 ± 412	106.5 ± 11.2
hOCT3	18.9 ± 2.4	1716 ± 157	27.4 ± 22.1	2421 ± 1233	37.1 ± 19.3
hMATE1	6.0 ± 0.7	487 ± 206	4.9 ± 1.4	975 ± 49	52.2 ± 16.2
hMATE2K	9.7 ± 4.1	2239 ± 385	19.3 ± 0.3	6102 ± 973	111 ± 27.6