TABLE 1.

Tolerant and intolerant residues from RGS domains of interest that also overlap with highly deleterious somatic mutations were selected for assessment of their impact on RGS functions. Amino acids reported in the COSMIC cancer database were selected based on the criteria that were predicted to be pathogenic by the FATHMM score to be highly pathogenic. Scores above 0.5 are deleterious; scores ≥0.7 are classified as pathogenic.

	Pathogenic Score	Cancer Type	3DMTR (Score)
RGS14
S127P	0.91	Stomach Carcinoma	Tolerant (1.03)
D137Y	0.97	Prostate Carcinoma	Tolerant (0.93)
R173C	0.81	Large Intestine Carcinoma	Intolerant (0.50)
R173H	0.97	Large Intestine Carcinoma	Intolerant (0.50)
RGS10
S64T	0.96	Lung Carcinoma	Tolerant (0.91)
K89M	0.84	Thyroid Carcinoma	Intolerant (0.48)
RGS4
K125Q	0.97	Lung Adenocarcinoma	Intolerant (0.48)
E126K	1.00	Skin Melanoma	Intolerant (0.42)
E135K	1.00	Skin Carcinoma, Melanoma, Upper Aerodigestive Tract Carcinoma	Tolerant (1.14)