TABLE 2.

The 3DMTR analysis is a better predictor of key protein residues for functional impact of somatic mutations than 1DMTR Summary results of the multiple functional assessments performed to characterize the effects of the reported mutations. Overall, the 3DMTR analysis is a better predictor of intolerant protein regions than 1DMTR. With this information we can better predict which reported mutations will lead to a change-of-function phenotype.

RGS	Selected Mutation	1DMTR Score	CoF Prediction	3DMTR Score	CoF Prediction	CoF Effect	Matched 3DMTR
RGS14	S127P	1.04	No	1.03	No	GoF	x
	D137Y	1.08	No	0.93	No	GoF	●
	R173C	0.92	No	0.50	Yes	LoF	●
	R173H	0.92	No	0.50	Yes	LoF	●
RGS10	S64T	0.85	No	0.91	No	Mixed	x
	K89M	0.69	No	0.48	Yes	Mixed	x
RGS4	K125Q	0.94	No	0.48	Yes	LoF	●
	E126K	0.99	No	0.42	Yes	LoF	●
	E135K	1.22	No	1.14	No	WT	●

●, matched prediction; x, no match.