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Method comparison schematic and calibration curves. (A) Outline of the method comparison experiment. (B) Standard curves were generated using the PicoProbe™ assay kit. The PicoProbe™ assay standards were prepared per the manufacturer’s recommendations. A set of standards also was prepared at the same concentrations using the stock standard used for the LC-MS assays. (C) Standard curves were generated via LC-HRMS and LC-MS/MS.
