TABLE 2.
Strains, plasmids, and primers used in this study
| Strain, plasmid, or primer | Relevant genotype, features, or sequence (5′−3′) | Reference |
|---|---|---|
| Strains | ||
| DH5αΤ1Ρ | ϕ80 fhuA dΔ(lxc-argF) [ΔlacZM15] U169 phoA supE44 gyrA96 recA1 relA1 endA1 thi1 hsdR17(rK− mK+) | Thermo Scientific |
| RY34010 | MG1655 ΔlacIZYA <>frt | TB28 in (56) |
| RY34348 | RY34010 ΔlysA763 <>frt | (57) |
| Plasmids | ||
| pBAD24 | bla araC ParaBAD | (58) |
| pBAD24_L | bla araC ParaBAD::L | (8) |
| pBAD24_E | bla araC ParaBAD::E | Laboratory collection |
| pBAD24_A2 | bla araC ParaBAD::A2 | This work |
| pBAD24_PP7 | bla araC ParaBAD::SglPP7 | Laboratory collection |
| pBAD24_KU1 | bla araC ParaBAD::SglKU1 | Laboratory collection |
| pBAD24_M | bla araC ParaBAD:: SglM | Laboratory collection |
| pBAD24_PRR1 | bla araC ParaBAD:: SglPRR1 | Laboratory collection |
| pAJEXD_Hgal | eilR PJExD :: SglHgal | This work |
| Primers | ||
| KC30 | cggcagaaaagtccacattg | |
| KC31 | cacactaccatcggcgcta | |
| KC710 | ggcgatagtgcgaaccttgtcattattgactttctg | |
| KC711 | gcccgagatattttatagtctcgagcccttcgcc | |
| KC713 | ggtgaaagatttgagccggatgtcacgtctgtc | |
| FA_A2_F_Ecor1 | gagagtgaattccatatgcctaaattaccgcgtgg | |
| FA_A2_R_Hind3 | gtatataagcttatcaacgctttacgcgttggg | |
| Forward pAJEXD_FA | ggatccaaactcgagtaaggatctcc | |
| Reverse pAJEXD_FA | atgtatatctccttcttaaaagatcttttgaattcaaagttgg | |
| FW Hgal_pAJEXD_FA | actttgaattcaaaagatcttttaagaaggagatatacatatggccaacccgcgc | |
| RV Hgal_pAJEXD_FA | tttatttgatgcctggagatccttactcgagtttggatccttacgtcatgggaaccacataggcgt | |