Fig. 2.

Hydrolysis of RBM1-151 in intact Farber (A) and melanoma (B, C) cells. The indicated cells were incubated with RBM1-151 (20 μM) for 3 h and further processed as indicated in the Materials and methods section. Data (mean ± SD) are from three experiments with triplicates. B: Asterisk indicates statistically significant difference at P < 0.0001 (unpaired, two tailed t-test between vehicle and SACLAC for each cell line). C: Different symbols denote statistically significant difference at P < 0.0001 by one-way ANOVA followed by Tukey multiple comparison test. A375/AC, AC-overexpressing A375 cells; A375/WT, A375 cells with AC basal levels; FD, Farber cells; FD/AC, Farber cells transduce to overexpress AC; SACLAC, (2S,3R)2-chloro-N-(1,3-dihydroxyoctadecan-2-yl)acetamide.