FIGURE 5.

Characterization of in vitro polarized TAMs. A, Cytokines and chemokines detected in the supernatant of high-density VSCC cultures by ELISA. B, Healthy donor monocytes were differentiated into macrophages using GMCSF and exposed to different culture conditions: medium alone (monocyte-derived macrophage), VSCC supernatant (in vitro polarized TAM), or 20 ng/mL IFNγ (M1). Flow cytometry on day 5 determined surface antigen expression, presented as d-gMFIs. C, Macrophages were generated as in B; TNFα and IL10 levels were quantified in the supernatant by ELISA on day 5; background levels were subtracted. Data represent results from two individuals and/or experiments and are presented as mean ± SEM; significance was determined by two-sided Student t test.