Figure 4.

Single-shot label-free multimodal nonlinear imaging (SHG, THG, CARS, 2PF, 3PF). Using a single fiber-laser pumped photonic crystal fiber source to generate supercontinuum illumination, along with parallel multi-channel photomultiplier tube detectors, multiple nonlinear processes can be excited in tissue simultaneously and detected to generate a spatially and temporally co-registered label-free image of tissue microstructure, molecular composition, function, and metabolism. Adapted from Ref. 56. Multimodal images, radar plots, and spectra for normal and tumor tissues can be found in Ref. 57.