Fig 5. Kismet mutants do not show changes in endocytosis when CME is inhibited.

Endocytosis was assessed by measuring internalization of the lipophilic dye FM 1-43FX after one min stimulation with 90 mM KCl. Larvae were pretreated with either 200 μM Chlorpromazine or 100 μM Dynasore to inhibit CME. (A) Panels show high resolution confocal micrographs of terminal presynaptic motor neuron boutons (HRP, magenta) after internalization of the lipophilic dye FM 1-43FX (green). (B) Data for each condition were normalized to w¹¹¹⁸ controls. Bars indicate means, points represent individual larvae, and error bars represent SEM. Endocytosis was unchanged in kis mutants pretreated with Chlorpromazine or Dynasore. Scale bar = 5 μm.