Fig 6. Kismet helps maintain vesicle pools.

Representative responses (top) in controls (n = 9) and kis mutants (n = 10) to 3 (A) or 10 (B) Hz stimulation in the presence of 2 μM Bafilomycin, which inhibits vesicle refilling. Muscles were clamped at -60 mV and recordings were obtained in HL-3 + 1.0 mM Ca²⁺. 3 Hz stimulation depletes the readily releasable and recycling pool of vesicles while 10 Hz stimulation deplete the reserve pool of vesicles. Vesicle pools are smaller in kis mutants as indicated by reduced eEJCs induced by stimulation. Bottom graphs show mean eEJC amplitudes normalized to the first stimulus for each condition. Error bars represent the SEM.