Fig 9. Expression of an ATPase deficient Kis, Kis^K2060R, in kis mutants restores behavior and endocytosis.

Tissue-specific drivers were used to express Kis^K2060R in all tissues (using the Actin5c-Gal4 driver), presynaptic motor neurons (using the elav-Gal4 driver), or postsynaptic muscle (using the 24B-Gal4 driver) of kis mutants. A) Histograms show quantification as determined by wrMTrck of larval crawling behavior on an agar arena for 30 s. Distance traveled, maximum velocity, average velocity, and crawling velocity normalized to body size (body lengths per second). B) Representative traces of larval crawling behavior for the genotypes listed. C) Quantification of FM 1-43FX fluorescence intensity indicates that expression of Kis^K2060R restores endocytosis in kis mutants. D) High resolution confocal micrographs of terminal presynaptic motor neuron boutons (HRP, magenta) after internalization of the lipophilic dye FM 1-43FX (green) after one min stimulation with 90 mM KCl in genotypes as listed. Scale bar = 5 μm.