FIG. 4.

Genomic organization of toroviruses and a schematic outline of the strategies used for amplification and sequencing of the 3′ genomic region. The upper panel shows a schematic representation of the torovirus genome, with the genes represented by boxes. The genes for the polymerase (Pol 1a, Pol 1b), the spike protein (S), the membrane protein (M), the hemagglutinin-esterase (HE), and the nucleocapsid protein (N) are indicated. The lower panel shows a schematic outline of the RT PCR assay targeted to the NTR and the N-protein gene. The positions and orientations of the oligonucleotides on the PoTV genome are shown, as are the lengths of the products of the PCRs. Primer 593 was used only in the sequence analysis.