Table 6.
Immune response and oxidative stress status in the duodenum, jejunum, and plasma of nursery pigs fed with β-mannanase in experiment 2.
| Item1 | β-mannanase, U2/kg | SEM | P value | |||
|---|---|---|---|---|---|---|
| 0 | 400 | 600 | Linear | Quadratic | ||
| Duodenum | ||||||
| TNF-α, pg/mg protein | 5.81 | 5.19 | 3.81 | 0.58 | 0.033 | 0.342 |
| IgG, μg/mg protein | 1.44 | 1.45 | 1.07 | 0.20 | 0.038 | 0.081 |
| MDA, nmol/mg protein | 1.46 | 1.31 | 1.26 | 0.10 | 0.046 | 0.859 |
| PC, nmol/mg protein | 3.71 | 3.89 | 3.22 | 0.30 | 0.249 | 0.093 |
| Jejunum | ||||||
| TNF-α, pg/mg protein | 6.23 | 5.51 | 4.19 | 0.79 | 0.046 | 0.438 |
| IgG, μg/mg protein | 1.20 | 1.07 | 0.80 | 0.10 | 0.008 | 0.250 |
| MDA, nmol/mg protein | 1.06 | 0.96 | 0.69 | 0.12 | 0.002 | 0.123 |
| PC, nmol/mg protein | 7.11 | 4.61 | 4.22 | 0.41 | <0.001 | 0.268 |
| Plasma | ||||||
| TNF-α, pg/mL | 167.4 | 69.9 | 81.0 | 19.8 | <0.001 | 0.071 |
| IgG, mg/mL | 1.54 | 1.35 | 1.03 | 0.12 | 0.004 | 0.275 |
| MDA, nmol/mL | 19.8 | 11.9 | 17.6 | 2.2 | 0.187 | 0.011 |
| PC, nmol/mg protein | 3.76 | 3.33 | 3.68 | 0.44 | 0.737 | 0.375 |
1TNF-α: tumor necrosis factor-α; IgG: immunoglobulin G; MDA: malondialdehyde; PC: protein carbonyl.
2One unit of β-mannanase activity is defined as the amount of enzyme required to releases 1 μmol of mannose reducing sugars equivalents per minute from locust bean gum (1.0%) in sodium phosphate buffer (200 mM), pH 6.0 at 50°C.