Table 7.
Morphology and crypt cell proliferation in the duodenum and jejunum of nursery pigs fed with β-mannanase in experiment 2.
| Item | β-mannanase, U1/kg | SEM | P value | |||
|---|---|---|---|---|---|---|
| 0 | 400 | 600 | Linear | Quadratic | ||
| Duodenum | ||||||
| Villus height, μm | 579 | 671 | 651 | 43 | 0.023 | 0.174 |
| Crypt depth, μm | 281 | 303 | 301 | 8.1 | 0.042 | 0.329 |
| VH:CD2 | 2.06 | 2.20 | 2.15 | 0.12 | 0.313 | 0.379 |
| Jejunum | ||||||
| Villus height, μm | 426 | 487 | 516 | 16 | <0.001 | 0.944 |
| Crypt depth, μm | 175 | 224 | 246 | 7.8 | <0.001 | 0.864 |
| VH:CD | 2.48 | 2.19 | 2.13 | 0.11 | 0.022 | 0.687 |
| Crypt cell proliferation, % | 29.3 | 40.7 | 35.5 | 1.99 | 0.014 | 0.011 |
| Tight junction proteins3 | ||||||
| Occludin | 0.80 | 0.72 | 0.88 | 0.09 | 0.577 | 0.294 |
| Claudin-1 | 0.57 | 0.52 | 0.54 | 0.18 | 0.791 | 0.772 |
| Zonula occludens -1 | 0.84 | 0.84 | 1.18 | 0.13 | 0.083 | 0.307 |
1One unit of β-mannanase activity is defined as the amount of enzyme required to releases 1 μmol of mannose reducing sugars equivalents per minute from locust bean gum (1.0%) in sodium phosphate buffer (200 mM), pH 6.0 at 50°C.
2VH:CD:villus height to crypt depth ratio.
3Relative band density represents each tight junction’s band ratio compared with β-action’s band density.