Table 2.
Primary antibodies used in this study
| Antigen | Immunizing species | Vendor (primary) | Antibodies dilution used | Secondary antibodies dilution used | Vendor (secondary) | Purpose | RRID |
|---|---|---|---|---|---|---|---|
| Tyrosine hydroxylase (TH) | Mouse | Millipore, catalog #MAB318 | 1:1,000 | Horse anti-mouse 1:200 (biotinylated) | Vector, catalog #BA2000 | To identify dopamine- containing neurons and terminals | AB_2201528 |
| Calbindin D28k (Cb) | Goat | R&D Systems, catalog #AF3320 | 1:1,000 | Horse anti-goat 1:200 (biotinylated) | Vector, catalog #BA9500 | To delineate thalamic nuclei | AB_2254256 |
| GFP | Rabbit | Life Technologies, catalog #A11122 | 1:5,000 | Goat anti-rabbit 1:200 (biotinylated) | Vector, catalog #BA9400 | To identify EYFP-positive elements (EYFP is a variant of GFP) | AB_221569 |