Figure 2—

Representative results of western blot analysis for 5-lipoxygenase in SCCF1 and A-72 thymic fibroblast lysates, compared with 2 μg of recombinant his-5-lipoxygenase (rhis–5-LOX) and mean ± SD viability of SCCF1 cells treated for 48 hours with various concentrations of piroxicam, TAM, licofelone, tepoxalin, and tepoxalin with TAM, or a vehicle control (DMSO). For the western blot analysis, 30 μg of each lysate was loaded into each lane. Cell viability was determined by use of MTT assays, and results are expressed as a percentage of vehicle control–treated cell viability. *At this concentration, percentage viability of tepoxalin-treated and tepoxalin-TAM–treated cells differed significantly (P < 0.05) from the findings for the vehicle control–treated cells. †At this concentration, percentage viability of licofelone-treated cells differed significantly (P < 0.05) from the findings for the vehicle control–treated cells.