TABLE 2.
Neutralization of pseudotyped virus infectivity on BHK cells
| Antibody testeda | Neutralization withb:
|
|
|---|---|---|
| HCV E1/VSV | HCV E2/VSV | |
| Mouse antiserum to P1 peptide | 1/20 | ND |
| Mouse antiserum to P2 peptide | 1/20 | ND |
| MAb to E1 | 1/20 | ND |
| Rabbit antiserum to E1 (WU122) | 1/20 | <1/10 |
| Rabbit antiserum to E2 (WU105) | ND | <1/10 |
| Mouse antiserum to E2 (2m) | <10 | 1/100 |
| MAb to E2 | ND | <1/10 |
a
HCV-specific antibodies were tested at 1/10 and higher dilutions.
b
The indicated pseudotyped viruses were treated with hyperimmune rabbit antisera to VSV (1:50) and VV (1:50) as an additional precautionary step in case of reversion of VSV or leakage of VV during testing for neutralization with HCV-specific antibodies. ND, not determined.