Figure 1.

Additional experimental data gathered from multiple, heterogeneous sources were incorporated into the E. coli whole-cell model to simulate cells that transcribe polycistronic mRNAs. (A) Schematic representation of the data curation process that was used to update the whole-cell model. We incorporated additional data on E. coli’s transcription units into the existing whole-cell model, iteratively identified inconsistencies between the input data using the simulation outputs, and made guided corrections to the input data, which were verified through comparisons with independent data, to finalize an updated version of the E. coli whole-cell model that transcribes polycistronic mRNAs. (B) Comparison of the simulated expression dynamics of the frdABCD operon before and after the update.