Figure 4.

The LC is involved in processing information regarding environmental context

(A) LVST neurons labeled via CTB-647 injection into the lumbar spinal cord and sections stained with anti-TH. Higher magnification images (middle and right) show apposition of TH-positive enlargements in the vicinity of LVST neurons.

(B) Experimental procedure to test effect of noradrenergic neurotoxin DSP-4 on responses to lateral perturbations.

(C) Comparison of peak EMG amplitudes in TA after perturbation in control animals in high- and low-wall conditions.

(D) Comparison of peak EMG amplitudes in TA muscle after perturbation in animals following DSP-4 injection in high- and low-wall conditions.

(E) Experimental procedure for selective labeling of noradrenergic neurons in the LC using the PRSx8 promoter.

(F) TH immunostaining at site of injection of AAV described in (E).

(G and H) GFP labeling and (H) merge of same site shown in (F).

(I) Proportion of TH-positive neurons in the LC that express GFP following AAV injection.

(J) Proportion of TH-negative neurons expressing GFP.

(K) Experimental strategy for blocking synaptic transmission from neurons in the LC.

(L) Bilateral targeting of TeLC-GFP to the LC.

(M) Higher magnification of image in (L).

(N) Peak EMG responses of TA muscle in response to perturbations in high- or low-wall conditions in animals expressing GFP in the LC.

(O) Peak EMG responses of TA muscle in response to perturbations in high- or low-wall conditions in animals expressing GFP-TeLC in the LC.

Error bars are ± SEM