Figure 3.

Differentiation into MPECs is hindered in a priming environment deficient in GPR41 and GPR43. Ffar2^–/–;Ffar3^–/– and WT recipients were transferred 5 × 10⁴ naïve WT gBT-I cells and subsequently epicutaneously infected with 1 × 10⁶ PFU HSV-1. (A, B) gBT-I cells were analysed 9 days after infection for SLEC and MPEC proportions in the spleen (A). (B) IFN-γ and TNF-α were measured in gBT-I cells from the spleen following re-stimulation with gB_498-505 peptide for 5 hours in the presence of brefeldin A. GzmB and perforin expression was analysed without re-stimulation. (C) Flank skins were harvested 5 days after HSV-1 infection to measure viral titres using PFU assays. Data are presented as representative contour plots or bar graphs with mean + SEM of n = 8 – 10 mice per group from 2 independent experiments. Asterisks indicate statistically significant differences as analysed by Student’s t-tests (non-significant (ns) p ≥ 0.05, **p < 0.01, and ***p < 0.001).