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. 2024 Mar 8;11:1349417. doi: 10.3389/fcvm.2024.1349417

Table 1.

DNA primer sequences and UPL probes used in the Quantitative real-time PCR.

Gen Name Primer F (5’-3’) Primer R (5’-3’) UPL probe
MPC1 Mitochondrial pyruvate carrier 1 AGGATTTCCGGGACTACCTC GGAAGACCCCAGTTGGCTAC #57
MPC2 Mitochondrial pyruvate carrier 2 ATGCTGCCCGAGAAATTG TGGAGCCCAGAAGAAAACTG #62
GAPDH Glyceraldehyde 3-phosphate dehydrogenase AGCCACATCGCTCAGACAC GCCCAATACGACCAAATCC #45
HPRT1 Hypoxanthine Phosphoribosyltransferase TGACCTTGATTTATTTTGCATACC CGAGCAAGACGTTCAGTCCT #22
ACTB Actin beta AGAGCTACGAGCTGCCT GAC GGATGCCACAGGACTCCA #9

F, forward; R, reverse.