Figure 1.

Organ-on-Chip Design (A) The image shows the vascular channel network and inlets and outlets with tubing inserted. (B) Bright field image shows the vascular channels and tissue compartment of the organ-on-chip. The vascular channels and tissue compartment are connected by 3 µm pores. (C) HLMVEC grow to confluent to cover the vascular channels. F-actin is labeled green with phalloidin and nuclei labeled blue with Hoechst 33342. (Scale bar = 100 µm). (D) Spatial variations in flow conditions in the vascular networks and at bifurcations showing shear rates in different vessel in the network in the organ-on-chip. Blue indicates a low shear rate and red a high shear rate. The effects of shear flow and vessel geometry on neutrophil adhesion and migration can be determined in this system.