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. 2024 Mar 21;15:2531. doi: 10.1038/s41467-024-46857-w

Fig. 1. Identification of GAS41 as a ferroptosis repressor upon ROS stress.

Fig. 1

a Top hit of genomic-wide CRISPR-Cas9 screen in human melanoma A375 cells treated with TBH and Nutlin-3 were shown as a table. YEATS4 was highlighted in red. b Western blot of GAS41 and SLC7A11 protein levels in sgNC and sgGAS41 A375 cells generated using control sgRNA and two individuals targeting GAS41 sgRNA, respectively. c Cell viability of sgNC and sgGAS41 A375 cells treated with TBH for 6 h. d Cell viability of sgNC and sgGAS41 A375 cells treated with IKE for 24 h. e Western blot of GAS41 protein levels of sgNC and sgGAS41 A549 cells generated using control sgRNA and two individuals targeting GAS41 sgRNAs, respectively. f Cell viability of sgNC and sgGAS41 A549 cells treated with TBH for 6 h. g Assessment of lipid peroxidation by flow cytometry after C11-BODIPY staining of sgNC and sgGAS41 A549 cells treated with TBH (120 μM) and Ferr-1 (2 μM) for 4 h as indicated. h Western blot of GAS41 protein levels in sgNC and sgGAS41 H460 cells generated using control sgRNA and two individuals targeting GAS41 sgRNA, respectively. i Cell viability of sgNC and sgGAS41 H460 cells treated with TBH for 6 h. j Assessment of lipid peroxidation by flow cytometry after C11-BODIPY staining of sgNC and sgGAS41 H460 cells treated with (120 μM) and Ferr-1 (2 μM) for 4 h as indicated. k Cell viability of sgNC and sgGAS41 A549 cells treated with RSL-3 for 24 h. Data are mean ± SD of n = 3 independent biological repeats. p values were calculated using unpaired, two-tailed Student’s t test. Western blot experiments above (b, e, and h) were repeated three times with similar results and representative images are shown. Source data are provided as a Source Data file.