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. 2024 Mar 21;7:349. doi: 10.1038/s42003-024-06012-z

Table 2.

Primers used in this study

Primer Sequence (5’ – > 3’) Description
qacA_Fp CCTCACTAAAGGGAACAAAAGCTGGGTACCCTTTTAATTCTAGCGTGCCTAC Flanking primers used for amplification of gene cassettes for qacA deletion and complementation
qacA_Rp CGACTCACTATAGGGCGAATTGGAGCTCCGTAATTTAGAAATAATATTTATTGGTATTTCAAG
qacA_SOE_Fp CTAATCTACAATATCTAAAAATATATGTTTAGTATTAAGTTCCCTCCAATCCTTATAG Construction of gene cassette for qacA deletion by Splicing by Overlap Extension PCR
qacA_SOE_Rp CTATAAGGATTGGAGGGAACTTAATACTAAACATATATTTTTAGATATTGTAGATTAG
qacA_comp_Fp CTATTACAACCCACGGAATAATATCTGCTAGTC Construction of gene cassette for qacA complementation by Splicing by Overlap Extension PCR
qacA_comp_Rp GACTAGCAGATATTATTCCGTGGGTTGTAATAG