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. 2024 Mar 21;14:6777. doi: 10.1038/s41598-024-56845-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of the cells isolated from dental pulp tissues and gingiva. (a) Evaluation of stem cell surface markers using flow cytometry. Multi-lineage differentiation potential toward (b) osteogenic and (c) adipogenic lineage. Proteomic profile of decellularized extracellular matrix derived from human dental pulp stem cells (dECM_DPSCs) and gingival-derived mesenchymal stem cells (dECM_GSCs) demonstrated that the signature proteins of dECM_DPSCs and dECM_GSCs were members of the osteogenic-associated pathways. (d) Venn diagram of quantified common proteins and unique proteins from dECM_DPSCs and dECM_GSCs. The analyzes were performed using an interactive Venn diagram viewer⁴⁷. All proteins of each cell were analyzed and classified as (e) core matrisome and (f) matrisome associated proteins, respectively, using the human matrisome database downloaded from http://matrisomeproject.mit.edu/other-resources/human-matrisome/⁴⁸.