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. 2024 Mar 21;14:6777. doi: 10.1038/s41598-024-56845-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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A diagrammatic representation of the experimental strategy. DPSCs and GSCs were isolated from pulp and gingival tissues collected from impacted permanent molars of healthy individuals. Cells were cultured in normal growth medium and osteogenic induction medium for 21 days to facilitate ECM production. Subsequently, decellularization was performed to obtain dECMs. A comprehensive proteomic analysis was conducted to assess the matrisome profiling of these dECMs. Subsequent experimentation involved the reseeding of GSCs onto each dECM, followed by culture in normal growth medium and osteogenic induction medium to elucidate the cellular responses and osteogenic differentiation capabilities of the reseeded cells. Furthermore, RNA sequencing techniques were employed to analyze differential gene expression, identify enriched pathways, and characterize GO terms associated with the experimental conditions. Created by biorender.com.