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. 2023 Dec 27;21(4):e14447. doi: 10.1111/iwj.14447

FIGURE 9.

FIGURE 9

Upregulated expression of C10orf99 in chronic wounds and WHC. Expression of C10orf99 (encoding GPR15L) was significantly increased in LUs, FUs, PUs and WHCs. (A) Levels of C10orf99 mRNA were aggregated from both wound border and bed subregions. Each bar represented the median level of C10orf99 expression for each ulcer type as shown in the legend, top right. Each dot represented the average C10orf99 expression for each subject. (B) Average C10orf99 levels of all ulcer types as shown in A were higher in the wound border compared to the wound bed. The graph highlighted the median expression levels of C10orf99 in each subregion, colour‐coded as shown in the legend on the top left. (C) Increased levels of GPR15L detected in chronic wounds and WHCs compared to intact skin. HE‐stained tissues representing each wound type and intact skin are shown on the right. Signal specificity was confirmed by the omission of the anti‐GPR15L antibody for each tissue type (middle panel). Tissue samples 1,2,3, intact skin (00099, visit 1); 4,5,6, LU (50 037, visit 1); 7,8,9, PU (50 051, visit 1); 10,11,12, FU (50 002, visit 1); 13,14,15, WHC (50 050, visit 2). Black scale bars, 500 μm; white scale bars, 100 μm. (D) Anti‐GPR15L staining of a full LU section revealed increased levels of immunoreactive protein in the wound border compared to the wound bed. Top, HE; bottom, IHC). Tissue samples 16,17, LU (50 037, visit 1). Black scale bar, 2500 μm; white scale bar, 1000 μm.