Table 2.
Reagents used in OMIP-101.
| Specificity | Fluorochrome | Clone | Purpose | |
|---|---|---|---|---|
| CD45 | BV785 | HI30 | Leukocytes | Lineage |
| CD66 | BV750 | B1.1 | Granulocytes | |
| CD33 | PE-Cy5 | WM53 | Myeloid cells | |
| CD14 | BB660 | MφP9 | Monocytes | |
| CD11c | BUV661 | B-ly6 | Myeloid dendritic cells | |
| CD19 | R718 | HIB19 | B cells | |
| CD56 | BV711 | HCD56 | NK cells, NKT-like cells | |
| CD127 | PE-Cy5.5 | eBioRD5 | ILCs, T cell differentiation | |
| CD3 | BUV496 | UCHT-1 | T cells | |
| CD4 | BV480 | SK3 | Helper T cells | |
| CD8 | BV570 | RPA-T8 | Cytotoxic T cells | |
| γδ TCR | BUV395 | B1 | γδ T cells | |
| TRAV1.2 | PE | 3C10 | MAIT cells | |
| CD161 | BV650 | DX12 | MAIT cells, T/NK cell differentiation | |
| CD38 | BB700 | HIT2 | Monocyte, mDC, T cell, and B cell activation/differentiation, plasmablasts | Phenotype / cell subsets |
| HLA-DR | BUV563 | G46-6 | Myeloid subsets, T cell activation | |
| CD32 | BB630 | FLI8.26 | Fc receptor | |
| CD16 | BV605 | 3G8 | Monocyte, NK, and mDC subsets | |
| IgD | BUV737 | IA6-2 | B cell differentiation | |
| CD27 | BUV805 | M-T271 | T, B, and NK cell differentiation | |
| CD57 | BB515 | NK-1 | NK and T cell differentiation | |
| CD45RA | APC-eFluor 780 | HI100 | T cell differentiation | |
| CCR7 | PE-CF594 | 150503 | T cell differentiation | |
| Vδ2 | BB790 | B6 | γδ T cell subset | |
| Perforin | BV421 | γG9 | CD8, NK and NKT cell cytotoxicity | Function |
| Granzyme B | AF647 | DH2 | CD8, NK and NKT-like cell cytotoxicity | |
| Ki67 | PE-Cy7 | B56 | Proliferation | |