FIG. 5.

Identification of proviruses with partially deleted 5′LTR termini: Southern blotting and sequence analysis of the 5′LTRs of proviruses in SG1 PBMC. Hybridization with L2-F (A) and L3-R (B) ³²P-labelled probes is shown. Lanes −, negative controls (DNA extracted from 10⁵ PBMC of a healthy blood donor). Lanes 1 and 2, positive controls (1 pg [lane 1] and 1 fg [lane 2] of pNL4-3 DNA mixed with DNA extracted from 10⁵ PBMC of a healthy donor). The other lanes show PCR products of DNA extracted from 10⁵ PBMC collected from patient SG1 at the May 1995 and June 1996 time points. (C) Alignment of partial sequences relative to the 5′LTR regions of clones obtained from the 680-bp PCR product.