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Inhibition of trypsin with water-soluble coffee melanoidins. (A) Dose–response curve of trypsin and water-soluble coffee melanoidins. The dotted line marks the concentration present in the simulated intestinal digestion experiment (0.125 mg/mL). (B) Michaelis–Menten kinetics in the absence (−M) or presence of 0.08 mg/mL water-soluble coffee melanoidins (+M). Data are mean ± S.D., n = 3.
