Recovery
from inactivation is limited by compound unbinding. Plots
of normalized recovery from inactivation in NaV1.6 channels
induced by a 10s prepulse to 0 mV in the presence of different concentrations
of compounds at −120 mV measured using the protocol shown in
inset. (A) XPC-7224, n = 4–12 cells per concentration
(B) XPC-5462, n = 3–8 cells per concentration
(C) PHY, n = 6–17 cells per concentration
(D) CBZ, n = 3–13 cells per concentration.
(E) Time constants of the slow component of recovery induced by compound
binding against concentration of compound measured at 1.7 s. (F) Plot
of the fractional recovery after 1.7 s against compound concentration
for XPC-7224 (n = 78 cells), XPC-5462 (n = 63 cells), and PHY (n = 109 cells). Mean data
were fit with a double exponential function.