Figure 3.
Functional screening of carrageenan sulfatases in carrageenolytic polysaccharide utilization loci (PULs). (a) Sulfatases active on κ-carrageenan or oligo-κ-carrageenan produced by BovGH16 pretreatment. (b) Sulfatases active on ι-carrageenan or oligo-ι-carrageenan produced by BovGH16 pretreatment. The amount of released sulfate was determined by a previously developed sulfate assay.31 (c) Production of DA from 0.5% (w/v) κ-carrageenan and (d) production of DA from 0.5% (w/v) ι-carrageenan. For these experiments, the amount of released DA was determined by the action of the DA dehydrogenase from C. algicola DSM 14237. (e) Carrageenolytic clusters examined in this study. Descriptions over the arrows indicate the CAZy family (GH127, GH129) or sulfatase classes of the respective enzymes according to the SulfAtlas database.5