Table 2.

Sample reaction setup for divalent cation titrations (e.g., Mg²⁺, using a 1 M stock)

Final Mg2+ conc.	RNA (10–50 μM)	CEK buffer (10×)	MgCl2 (1 M)	H2O	Total volume
$X$ mM	${\rm Y}$ μL for (0.5 OD)	50 μL	0.5× μL	$[500-({\rm Y}+50+0.5X)]$ μL	500 μL