Fig. 8.

HOCl scavenger taurine inhibited HMGB1 secretion in rat ischemic brains during cerebral ischemia–reperfusion injury. I/R rats were subjected to 2 h of MCAO ischemia plus 24 h of reperfusion. Sham control rats were subjected to similar operations without MCAO. Taurine (Tau, 50 mg/kg) was intravenously administered at the onset of reperfusion. A Co-immunostaining detection of HOCl and HMGB1 location in I/R rat brains. B Immunofluorescent staining of HMGB1 in neurons of the ischemic cortical area. C, D Western blot and quantitative analysis for HMGB1 expression in I/R rat brains. E ELISA detection of HMGB1 secretion in rat plasma. *p < 0.05. All data are presented as mean ± SEM and each point denotes one animal. (Statistical methods: D one-way ANOVA followed by Tukey’s multiple comparisons test; E two-tailed t-test.)