TABLE 3.
Distribution of isotypes of rabies virus-specific antibody elicited by infection with CVS-F3
| Isotype | Geometric mean rabies virus-specific antibody titera in indicated mouse strain
|
||||
|---|---|---|---|---|---|
| 129/SvEv | IFN-α/βR k.o. | IFN-γR k.o. | C57BL/6J | β2m k.o. | |
| IgM | 5,649 (2,440–9,760) | 34,995 (17,920–71,680) | 37,670 (40,960–110,080) | 29,512 (25,320–32,805) | 21,135 (5,603–38,641) |
| IgG1 | 724 (0–40,960) | 40,551 (21,760–117,760) | 16,143 (3,520–125,440) | 29 (0–640) | 3 (0–130) |
| IgG2a | 444,631 (102,400–1,392,640) | 11,272 (9,122–24,762) | 45,920 (23,680–140,760) | 1,148 (586–1,813) | 92 (55–340) |
| IgG2b | 95,499 (27,520–547,840) | 40,738 (9,880–94,720) | 62,517 (29,440–209,920) | 7,244 (2,141–29,789) | 772 (480–2,382) |
| IgG3 | 0 (0–40) | 2 (0–110) | 0 | 1 (0–568) | 0 |
| IgA | 171,396 (38,560–7,536,640) | 17,219 (6,880–33,280) | 26,546 (3,040–79,360) | 876 (370–2,320) | 99 (0–5,920) |
a
Determined 20 days p.i. as the inverse of the last dilution of serum in ELISA which gave an optical density greater than twice the background optical density of wells with secondary isotype-specific antibody but without serum. Groups consisted of five mice each. Numbers in parentheses represent the range of antibody titers found within each group of animals. IFN-α/βR and IFN-γR k.o. mice are on a 129SvEv background, while β2m k.o. mice are on a C57/BL6 background.