Table 1. Primers and probes used for the molecular detection of respiratory viruses.

Virus type	Target region	Function	Sequence	Reference
RSV(A/B)	Matrix gene	Forward primer	5’-GGAAACATACGTGAACAAGCTTCA	[13]
		Reverse primer A	5’-CATCGTCTTTTTCTAAGACATTGTATTGA
		Reverse primer B	5’-TCATCATCTTTTTCTAGAACATTGTACTGA
		Probe	6FAM-TGTGTATGTGGAGCCTT- MGBNFQ
Adenovirus	Hexon gene	Forward primer	5’-GCCACGGTGGGGTTTCTAAACTT	[14]
		Reverse primer	5’-GCCCCAGTGGTCTTACATGCACAT
		Probe	6FAM-GCACCAGACCCGGGCTCAGGTACTCCGA-TAMRA
INF A	Matrix gene	Forward primer	5’- GACCRATCCTGTCACCTCTGAC	[15]
		Reverse primer	5’-AGGGCATTYTGGACAAAKCGTCTA
		Probe	5’-TGCAGTCCTCGCTCACTGGGCACG
INF B	Hemagglutin (HA) gene	Forward primer	5’-AAATACGGTGGATTAAATAAAAGCAA	[16]
		Reverse primer	5’-CCAGCAATAGCTCCGAAGAAA
		Probe	6FAM-CACCCATATTGGGCAATTTCCTATGGC- MGBNFQ
HRV	Non-coding region	Forward primer	5’-CPXGCCZGCGTGGC	[17]
		Reverse primer	5’-GAAACACGGCACCCAAAGTA
		Probe	5’-TCCTCCGGCCCCTGAATGYGGC
hMPV	Nucleoprotein	Sense	5’-CATCAGGTAATATCCCACAAAATCAG-3’	[18]
		Antisense	5’-GTGAATATTAAGGCACCTACACATAATAARA-3’	[18]
		Probe	6FAM-TCAGCACCAGACACAC-BBQ
OC43	Nucleoprotein	Forward primer	5’-CGATGAGGCTATTCCGACTAGGT	[19]
		Reverse primer	5’-CCTTCCTGAGGTAACC
		Probe	5’-TCCGCCTGGCCTCCCT
229E	Nucleoprotein	Forward primer	5’-CAGTCAAATGGATGCA	[19]
		Reverse primer	5’-AAAGGGCTATTATTCT
		Probe	5’-CCCTGACGACGGTTCA
NL63	Nucleoprotein	Forward	GACCAAAGCACTGAATAACATTTTCC	[19]
		Reverse	ACCTAATAAGCCTCTTTCTCAACCC
		Probe	6FAM-ATGTTATTCAGTGCTTTGGTCCTCGTGAT-BHQ1
		Probe	5’-TGTGTGGCGGAGCCTG
HKU1	Replicase gene	Forward	CCTTGCGAATGAATGTGCT	[19]
		Reverse	TTGCATCACCACTGCTAGTACCAC
		Probe	6FAM-TGTGTGGCGGTTGCTATTATGTTAAGCCTG-BHQ1
PIV 1	Polymerase gene	Forward primer	5’-ACAGATGAAATTTTCAAGTGCTACTTTAGT	[20]
		Reverse primer	5’-GCCTCTTTTAATGCCATATTATCATTAGA
		Probe	6FAM-ATGGTAATAAATCGACTCGCT- MGBNFQ
PIV 2	Polymerase gene	Forward primer	5’-TGCATGTTTTATAACTACTGATCTTGCTAA	[20]
		Reverse primer	5’-GTTCGAGCAAAATGGATTATGGT
		Probe	6FAM-ACTGTCTTCAATGGAGATAT- MGBNFQ
PIV 3	Matrix gene	Forward primer	5’-TGCTGTTCGATGCCAACAA	[20]
		Reverse primer	5’-ATTTTATGCTCCTATCTAGTGGAAGACA
		Probe	6FAM-TTGCTCTTGCTCCTCA- MGBNFQ