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. 2024 Mar 22;19(3):e0297796. doi: 10.1371/journal.pone.0297796

Fig 1. Analytical parameters of singleplex and multiplex qPCR/RT-qPCR assays for the detection of FRDC-associated pathogens and SARS-CoV-2.

Fig 1

A) Comparison of analytical sensitivity of each singleplex and multiplex qPCR/RT-qPCR assays for the detection of pathogens associated with FRDC. B) Analytical sensitivity determination of singleplex and multiplex qPCR/RT-qPCR assays. Each assay was performed using 12 replicates ranging from 103 to 100 copies/μl of IVT DNA/RNA. Each circle and square indicate the Ct value of one replicate obtained by singleplex and multiplex amplification, respectively. Short solid lines indicate the median Ct value and dashed lines indicate the detection limit. Ct: Cycle threshold; IVT RNA: in vitro transcribed RNA; R2: linearity; E: Efficiency; ND: not detected; NTC: no template control.