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. 2024 Mar 22;10(12):eadl4018. doi: 10.1126/sciadv.adl4018

Fig. 6. UM4118 induces cell death by cuproptosis.

Fig. 6.

(A) Representative images (left) of DLAT staining (red) in OCI-AML5 exposed 16 hours to 300 nM UM4118 or 20 nM elesclomol in media ± 1 μM copper. Nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI; blue). Quantification (right) of total DLAT signal divided per the number of cells in each image is depicted (unpaired t test compared to DMSO condition). (B) Intramitochondrial copper quantification by ICP-MS in human embryonic kidney 293 cells exposed 3 hours to indicated compounds. Data are represented as mean ± SD (n = 3, unpaired t test compared to DMSO condition). (C) Volcano plot representing the results of whole-genome CRISPR-Cas9 loss-of-function screen performed in EKO OCI-AML5 cells upon exposure to UM4118 (110 nM).