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. 2023 Nov 21;8(2):295–310. doi: 10.1093/evlett/qrad059

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© The Society for the Study of Evolution (SSE) and European Society for Evolutionary Biology (ESEN) 2023.

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Figure 8. — TAG content of germline-less vs. fertile control flies. The graph shows the TAG content (μg fat/ mg fly) of germline-ablated (sterile) female flies (red curve) vs. that of fertile control female flies (blue curve) as a function of adult age. Error bars represent standard errors of the mean. Starting at around 7 days of adult age, germline-less flies begin to harbor significantly higher levels of TAG levels than fertile flies. Analysis using a fully factorial two-way fixed-effects type II ANOVA revealed significant effects of Reproduction (germline-less vs. fertile control; F_1,116 = 28.5); Age (F_3,116 = 7.6); and Age × Reproduction (F_3,116 = 24.8); in all three cases, p < .001.