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. 2024 Mar 22;12:RP90989. doi: 10.7554/eLife.90989

Figure 4. Sporozoite expelling in relation to infection load in Anopheles coluzzii mosquitoes infected by naturally acquired gametocyte infections in Burkina Faso.

Figure 4.

(A) Direct feeding (blue) vs magnetic-activated cell sorting (MACS; green). Bars show the infection prevalence for each of the seven gametocyte carriers. Scatter plots with median lines show the midgut oocyst density as a result of direct feeding (blue) and MACS (green). (B) Binning of total sporozoite load and expelling prevalence (N = 25). (C) Scatter plot of absolute numbers of ruptured oocyst (sheet) density assessed by fluorescent microscopy vs total salivary gland sporozoite density assessed by COX-I qPCR; ρ = 0.90 (95% CI, 0.80–0.95). The line represents the fitted linear regression line and the gray shaded area is the 95% CI. (D) Scatter plot of absolute numbers of total sporozoite density (residual salivary gland sporozoites + sporozoites expelled) and sporozoites expelled into the artificial skin assessed by COX-I qPCR; ρ = 0.70 (CI, 0.52–0.82). The line represents the fitted linear regression line, the intercept is forced to zero for biological plausibility, and the gray shaded area is the 95% CI.

Figure 4—source data 1. Source data include re-analyses of correlation coefficients when different thresholds for data inclusion are imposed to determine whether associations are robust.