| Gene (Homo sapiens) |
Human vesicular monoamine transporter 2 |
Clone ID 40025175 |
NCBI Reference Sequence: BC108928
|
Horizon Discovery |
| Gene (Lama glama) |
Anti-GFP nanobody |
Plasmid #49172 |
|
Addgene |
| Cell line (Homo sapiens) |
HEK293S GnTI-
|
ATCC |
Cat # ATCC CRL-3022 |
Used for expression of VMAT2 (PMID:27929454) |
| Cell line (Spodoptera frugiperda) |
Sf9 |
ATCC |
Cat # ATCC CRL-1711 |
Used in production of baculovirus for transduction (PMID:27929454) |
| Transfected construct (human) |
pEG BacMam |
Gouaux lab |
|
PMID:25299155
|
| Affinity chromatography resin |
Strep-Tactin Superflow high capacity resin |
IBA Lifesciences |
Cat # 2-1208-500 |
Affinity purification resin |
| Chemical compound, drug |
n-Dodecyl-β-D-maltoside |
Anatrace |
Cat # D310 |
Detergent |
| Chemical compound, drug |
Cholesteryl hemisuccinate |
Anatrace |
Cat # CH210 |
Lipid |
| Chemical compound, drug |
Reserpine |
Sigma |
Cat # 83580 |
Inhibitor |
| Chemical compound, drug |
Dihydrotetrabenazine |
Cayman Chemicals |
Cat # 27182 |
Inhibitor |
| Chemical compound, drug |
[3H]5-HT |
PerkinElmer |
Cat # NET1167250UC |
Radiolabeled substrate |
| Chemical compound, drug |
[3H]dihydrotetrabenazine |
American Radiolabeled Chemicals |
Cat # ART 2119 |
Radiolabeled inhibitor |
| Chemical compound, drug |
Tetrabenazine |
Sigma |
Cat # T2952 |
Inhibitor |
| Chemical compound, detergent |
Digitonin |
Sigma |
Cat # 300410 |
Detergent |
| Software, algorithm |
Phenix |
PMID:22505256
|
RRID:SCR_014224
|
https://www.phenix-online.org/
|
| Software, algorithm |
SerialEM |
PMID:16182563
|
RRID:SCR_017293
|
http://bio3d.colorado.edu/SerialEM
|
| Software, algorithm |
CryoSPARC |
PMID:28165473
|
RRID:SCR_016501
|
https://cryosparc.com/
|
| Software, algorithm |
RELION |
PMID:23000701
|
RRID:SCR_016274
|
http://www2.mrc-lmb.cam.ac.uk/relion
|
| Software, algorithm |
UCSF-Chimera |
PMID:15264254
|
RRID:SCR_004097
|
https://www.cgl.ucsf.edu/chimera/
|
| Software, algorithm |
Coot |
PMID:15572765
|
RRID:SCR_014222
|
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot
|
| Software, algorithm |
MolProbity |
PMID:20057044
|
RRID:SCR_014226
|
http://molprobity.biochem.duke.edu/
|
| Other |
R 2/1 200 mesh Au holey carbon grids |
Electron Microscopy Sciences |
Cat # Q210AR1 |
Cryo-EM grids |
| Other |
R 1.2/1.3 200 mesh Cu holey carbon grids |
Electron Microscopy Sciences |
Cat # Q2100CR1.3 |
Cryo-EM grids |
| Other |
Copper HIS-Tag YSI |
PerkinElmer |
Cat # RPNQ0096 |
SPA beads |
