Table 1. Cryo-electron microscopy (cryo-EM) data collection, refinement, and validation statistics.
| VMAT2-TBZ(EMDB-41269)(PDB 8THR) | |
|---|---|
| Data collection and processing | |
| Magnification | 77,279 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 60 |
| Defocus range (μm) | –1.0 to –2.5 |
| Pixel size (Å) | 0.647 |
| Symmetry imposed | C1 |
| Initial particle images (no.) | ~10,000,000 |
| Final particle images (no.) | 65516 |
| Map resolution (Å) FSC threshold |
3.12 0.143 |
| Map resolution range (Å)* | 6.6–2.8 |
| Refinement | |
| Initial model used (PDB code) | |
| Model resolution (Å) FSC threshold |
3.7 0.5 |
| Model resolution range (Å) | 20.4–3.7 |
| Map sharpening B-factor Å2 | |
| Model composition Non-hydrogen atoms Protein residues Ligands |
2937 389 1 |
| B-factors Å2 Protein Ligand |
28.99 55.22 |
| R.m.s. deviations Bond lengths (Å) Bond angles (°) |
0.0004 (0) 0.37 (15) |
| Validation MolProbity score Clashscore Poor rotamers (%) |
1.23 4.34 0.96 |
| Ramachandran plot Favored (%) Allowed (%) Disallowed (%) |
98.0 2.00 0.00 |
*
Local resolution range at 0.5 FSC.