| Strain, strain background (Mus musculus) |
C57Bl/6 |
Jackson Laboratory |
Stock #: 000664; RRID:IMSR JAX:000664
|
|
| Genetic reagent (M. musculus) |
Plp1-Cre-ERT2 |
Leone et al., 2003
|
MGI:2663093
|
|
| Antibody |
anti-Yap/Taz (rabbit monoclonal) |
Cell Signaling Technology |
D24E4, #8418 RRID:AB_10950494
|
IHC 1:200 Western 1:1000 |
| Antibody |
anti-SCG10 (rabbit monoclonal) |
Novus Biologicals |
NBP1-49461 RRID:AB_10011569
|
IHC 1:5000 |
| Antibody |
anti-Yap (rabbit monoclonal) |
Cell Signaling Technology |
D8H1X, #14074 RRID:AB_2650491
|
IHC 1:200 |
| Antibody |
anti-Sox10 (goat polyclonal) |
R&D Systems |
#AF-2864 RRID:AB_442208
|
IHC 1:100 |
| Antibody |
anti-Sox10 (rabbit monoclonal) |
Abcam |
EPR4007, #ab155279 RRID:AB_2650603
|
IHC 1:250 |
| Antibody |
anti-Egr2 (rabbit polyclonal) |
Professor Dies Meijer, University of Edinburgh |
|
IHC 1:4000 |
| Antibody |
anti-Oct6 (rabbit monoclonal) |
Abcam |
EP5421, #ab126746 RRID:AB_11130256
|
WB 1:1000 |
| Antibody |
Anti-Oct6 (rabbit polyclonal) |
Abcam |
#ab31766 RRID:AB_776899
|
IHC 1:800 |
| Antibody |
Anti-c-Jun (mouse monoclonal) |
BD Transduction Laboratories |
#610326 RRID:AB_397716
|
IHC 1:500 |
| Antibody |
Anti-c-Jun (rabbit monoclonal) |
Cell Signaling Technology |
60 A8, #9165 RRID:AB_2130165
|
WB 1:1000 |
| Antibody |
Anti-pS63-c-Jun (rabbit polyclonal) |
Cell Signaling Technology |
#9261 RRID:AB_2130162
|
IHC 1:100 |
| Antibody |
anti-Ki67 (rabbit polyclonal) |
Abcam |
#ab15580 RRID:AB_443209
|
IHC 1:200 |
| Antibody |
anti-p75NGFR (goat polyclonal) |
Neuromics |
#GT15057 RRID:AB_2737189
|
IHC 1:400 |
| Antibody |
anti-Tubulin β3 (rabbit polyclonal) |
Biolegend |
#802001 RRID:AB_2564645
|
IHC 1:1000 |
| Antibody |
IRDye-680 (goat anti-mouse) |
LI-COR |
#926–32220 RRID:AB_621840
|
WB 1:15,000 |
| Antibody |
HRP-Goat anti-mouse secondary antibody |
Jackson Immunoresearch |
#715-035-150 RRID:AB_2340770
|
WB 1:12,000 |
| Antibody |
HRP-Goat anti-rabbit secondary antibody |
Jackson Immunoresearch |
#115-055-062 RRID:AB_2338533
|
WB 1:12,000 |
| Chemical compound, drug |
Araldite 6005 |
EMS |
#10920 |
|
| Chemical compound, drug |
DDSA |
EMS |
#13710 |
|
| Chemical compound, drug |
DBP |
EMS |
#13101 |
|
| Chemical compound, drug |
BDMA |
EMS |
#11400–25 |
|
| Other |
Coated grids (100 mesh) |
EMS |
#FF100-Cu |
|
| Chemical compound, drug |
Osmium tetroxide (4% solution) |
EMS |
#19170 |
|
| Chemical compound, drug |
Lead nitrate |
EMS |
#17900 |
|
| Chemical compound, drug |
Sodium citrate |
EMS |
#21140 |
|
| Chemical compound, drug |
Uranyl acetate |
EMS |
#22400 |
|
| Chemical compound, drug |
Sodium borate |
EMS |
#21130 |
|
| Chemical compound, drug |
Toluidine blue |
EMS |
#22050 |
|
| Chemical compound, drug |
Paraformaldehyde |
Sigma-Aldrich |
#158127 |
|
| Commercial assay or kit |
Click-It EdU Alexa Fluor 594 kit |
ThermoFisher Scientific |
#C10339
|
|
| Chemical compound, drug |
EdU |
ThermoFisher Scientific |
#E10187
|
|
| Chemical compound, drug |
Tamoxifen |
Sigma-Aldrich |
#T5648 |
|
| Other |
DAPI stain |
Invitrogen |
#D1306 |
IHC 1:250 |
| Antibody |
Alexa 488, 568 or 647 secondaries |
Jackson Immunoresearch |
|
IHC 1:250 to 1:1000 |
| Software, algorithm |
Image Studio Lite |
LI-COR, Inc |
|
|
| Software, algorithm |
Prism |
GraphPad Software, Inc |
|
|
| Software, algorithm |
Stata |
StataCorp LP |
|
Mann-Whitney test |
