Figure 3.

Effects of RhoA inhibition on proliferation of intestinal epithelial cells

(A–D) Two- and 17-month-old mice were intraperitoneally (i.p.) injected with 100 mg/kg 5-Bromo-2′-deoxyuridine (BrdU) 2 h before being euthanized. The number of cells in the S-phase of the cycle during this time was assessed by anti-BrdU immunohistochemistry (A and B; Scale bar: 50 μm). The average number of proliferative cells in the small intestinal epithelium of RhoA^T19N (Vil-Cre⁺;RhoA^T19N) and control (Vil-Cre⁻;RhoA^T19N) mice at the age of 2 months (C) and 17 months (D) is shown. At least 20 crypts from at least 7 animals per group were scored.

(D and E) The number of proliferating cells in small intestinal organoids derived from RhoA^T19N and control mice was assessed by flow cytometry after EdU (5-ethynyl-2′-deoxyuridine) staining. Representative results are shown in (D) and the quantification of three independent experiments is shown in (E). N = number of animals per group. The mean ± SEM is shown.