Figure 4.

Mitotic spindle orientation in small intestinal epithelial cells

(A–C) The orientation of the mitotic spindle of epithelial cells from the small intestine of control (Vil-Cre⁻;RhoA^T19N; A) and RhoA^T19N (Vil-Cre⁺;RhoA^T19N; B) mice was determined in hematoxylin and eosin-stained sections by measuring the angle (alpha) formed by the mitotic cell in metaphase or anaphase (dotted red line), and the epithelial surface (solid red line; C). Scale bar: 10 μm.

(D) The percentage of mitotic cells with angles in the indicated intervals is shown for control and RhoA^T19N mice. At least 140 mitosis were scored in at least 8 animals per group. Fisher’s exact test of alpha >30° in control vs. RhoA^T19N (5% and 25.2%, respectively), ∗∗∗p = 1.5 × 10⁻⁶.